The present study aims to investigate how English majors in China use the alignment principle while expressing disagreement by analyzing their ways of disagreement and the effects of proficiency on disagreement． The dyadic conversations of 48 subjects were selected as samples for data analysis from SECCL ( Spoken English Corpus of Chinese Learners) ． The ways that the subjects of different proficiency levels used to express disagreement were categorized and tallied． It was found that when the students expressed disagreement ，they chose to use significantly more alignment ways than non-alignment ways ，which confirmed the hypothesis that alignment was an important mechanism of conversation． Moreover ，compared to low achievers ，high achievers showed more variety in expressing disagreement and they were inclined to use more alignment strategies．