The present paper investigates perception and comprehension of accented speech produced by 30 Chinese non- English-Major postgraduates，through the judgment of 38 English-Major postgraduates and 20 oversea students from UK and USA． The study attempts to investigate the perception of accentedness by the Chinese and English listeners and the interference of accentedness in the comprehension of speech． Ｒesearch results show that prosodic features play the most important role in the perception of accentedness and there are certain differences between Chinese and English listeners in the relationship of accentedness and speech comprehension． This research sheds some light on second language phonetic acquisition and pronunciation teaching and learning．