On the basis of the theory of relevance and transfer, this study puts forward interpreting operation synergism through positive transfer from Visual-Audio-Oral Course of EAP to E-C & C-E interpreting. The purpose is to test the transferring effects from Visual-Audio-Oral Course of EAP to interpreting and to make student interpreters develop their interpreting synergism. In the way of consecutive interpreting, the researcher has done the experiments to 147 undergraduate interpreters of non-English majors in 5 classes taught by him in the second semester from 2014 to 2015. The analyses conducted on the big data through the method of tracking test, comparison and SPSS show that listening of EAP has the functions of relevance and holistic presupposition to student interpreters, whose audio nerves have been activated once more while listening to SL information. At this moment the function of transferring SL information as input occurs. And further, speaking of EAP also has the functions of relevance and holistic presupposition to student interpreters, whose oral nerves have been activated once more while encoding and expressing in TL. At this moment the results of transferring information as TL output are prominent. Therefore, Visual-Audio-Oral Course of EAP has the effect of relevance, transfer and presupposition to student interpreters’ interpreting operation.