Interrogation and focus are two important grammatical categories, and they are intrinsically linked. This paper starts with interrogative expression means, and analyzes the relationship between interrogation and focus from a cross-language perspective. Research found that different interrogative expression means have different effects on the determination of interrogative focus, that is, phonetic means can’t determine the focus of the interrogative sentence. Lexical means indicate special questions and can determine the focus of the interrogative sentences. The situation of grammatical means is quite complicated and English, French and Chinese have some similarities and differences. The relationship between interrogation and focus is reflected in a two-way interactive relationship.