The scientific and technological translation in the late Ming Dynasty was a climax in the history of Chinese translation, marking the beginning of the systematic exchange and integration of Chinese and Western scientific and technological cultures. The representative practices of this kind were those conducted by Matteo Ricci and Xu Guangqi. Taking Matteo Ricci’s cultural adaptation strategy as the object of examination, this paper explores the cultural integration in the practice of scientific and technological translation in the late Ming Dynasty. It is found at first that the social changes and unique cultural background of the late Ming Dynasty contributed to the formation of Ricci’s cultural adaptation strategy, and the development of western science and technology provided a rich practical basis for Ricci’s cultural adaptation strategy in a variety of translation practices. At the same time, the academic missionary work based on translation of Western science and technology, the localization policy showing his respect to Chinese culture, and the upper-class approach of befriending the scholars and bureaucrats were the main elements of the cultural adaptation strategy. On the basis of understanding the strategy of cultural adaptation, the paper, thereafter, analyzes the cultural integration in the translated works of the late Ming Dynasty represented by Elements of Euclid, Tongwen Suanzhi (Epitome Arithmetic Practice), and Qiankun Tiyi, among others. It is finally concluded that the cultural adaptation strategy facilitated the mutual learning and integration of Chinese and Western scientific and technological cultures in the late Ming Dynasty, thus promoting the transformation of the traditional Chinese scientific and technological knowledge system into a new knowledge system.